Description
For additional information
ask a questionDensitometer is designed for measurement of cell suspension's turbidity in the range of 0.0–6.0 McFarland units (0 – 180×107 cells/ml).
Densitometers provide the opportunity to measure solution turbidity in a wider range (up to 15.0 McFarland units) however, it is necessary to remember that in this case the standard deviation values increase.
A densitometer is used for measurement of cell concentration (bacterial, yeast cells) during fermentation process, determination of microorganism sensitivity to antibiotics, microorganism identification using various test-systems, for measurement of absorption at the definite wavelength, as well as for quantitative estimation of concentration of colour solution, absorbing green light.
The operation principle is based on measurement of optical density with digital presentation of results in McFarland units. The unit is calibrated at the factory (for operation with 16 mm diameter glass tubes) and keeps calibration without power supply. However, if necessary it is possible to calibrate the unit by 2–6 points in 0.0–6.0 McFarland unit range. We recommend to use Biosan standards to ensure full reliability, but it is acceptable to use other commercial as well as self prepared standards (e.g. BaSO4). Possibility to restore factory calibration settings.
Following calibration kits are available on request:
- CKG16 for glass tubes with diameter 16 mm, set of 0.5; 1.0; 2.0; 3.0; 4.0 McFarland Turbidity Standards (latex particles).
Cat.Nr.: BS-050102-BK - Calibration kit for glass tubes with diameter 18 mm, set of 0.5; 1.0; 2.0; 3.0; 4.0; 5.0 McFarland Turbidity Standards (BaSO4).
Cat.Nr.: 70900 - Calibration kit for glass tubes with diameter 12 mm, set of 0.0 (blank); 0.5; 2.0; 3.0 McFarland Turbidity Standards (latex particles).
Cat.Nr.: 21255
Two versions of the product are available:
- DEN-1 powered from external energy supply;
- DEN-1B powered both from external energy supply and from batteries (АА).
Measurement range | 0.00–15.00 McF |
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Display resolution | 0.01 McF |
Light source | LED |
Measurement wavelength (λ) | λ = 565 ±15 nm |
Accuracy (0.0–6.0 McF) | ±3% |
Measurement time | 1 s |
Sample volume | not less than 2 ml |
Tube external diameter | 12 mm, 16 mm (using A-12, A-16 adapter) or 18 mm (without adapter) |
Possibility to restore factory calibration settings | + |
Display | LCD |
Independent power supply | 3 × AA batteries |
Overall dimensions (W×D×H) | 165 × 115 × 75 mm |
Weight | 0.7 kg |
Input current/power consumption | 12 V, 7 mA / 0.1 W |
External power supply | Input AC 100–240 V, 50/60 Hz; Output DC 12 V |
Standard set | External power supply, A-16 and 3 × AA batteries |
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Can I use tubes of different diameter?
Yes. Glass and transparent plastic tubes (12, 16 or 18 mm in external diameter) can be used for work with DEN densitometers. A-12 (optional), A-16 adapter (included) must be used for work with tubes which are 12mm, 16 mm in external diameter.
Note! The unit must be calibrated every time a tube type (e.g. with different ext. diameter, bottom shape or different material) is changed.
Can standards prepared in the laboratory be used for calibration?
Yes. Both commercial standards and standards prepared in the laboratory can be used for calibration.
For what type of tubes is DEN-1/DEN-1B precalibrated at the factory?
From December 2015 factory calibration is for work with glass tubes with an external dimeter of 16 mm
How to calibrate DEN-1/DEN-1B?
Calibration procedure is described in the user manual p.6. See user manual in downloads section.
How to restore factory calibration settings?
Procedure of restoration of factory calibration settings is described in the user manual p 6.10. See user manual in downloads section.
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Glass Test Tubes 16x100mm, high borosilicate, PP Cap with silicone pad.
Packing - 100 pcs/boxCKG16 for glass tubes with diameter 16 mm, set of 0.5; 1.0; 2.0; 3.0; 4.0 McFarland Turbidity Standards (latex particles).
Glass Test Tubes 18x100mm, high borosilicate, PP Cap with silicone pad.
Packing - 100 pcs/box