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Densitometer is designed for measurement of cell suspension's turbidity in the range of 0.0–6.0 McFarland units (0 – 180×107 cells/ml).
Densitometers provide the opportunity to measure solution turbidity in a wider range (up to 15.0 McFarland units) however, it is necessary to remember that in this case the standard deviation values increase.
A densitometer is used for measurement of cell concentration (bacterial, yeast cells) during fermentation process, determination of microorganism sensitivity to antibiotics, microorganism identification using various test-systems, for measurement of absorption at the definite wavelength, as well as for quantitative estimation of concentration of colour solution, absorbing green light.
The operation principle is based on measurement of optical density with digital presentation of results in McFarland units. The unit is calibrated at the factory (for operation with 16 mm diameter glass tubes) and keeps calibration without power supply. However, if necessary it is possible to calibrate the unit by 2–6 points in 0.0–6.0 McFarland unit range. We recommend to use Biosan standards to ensure full reliability, but it is acceptable to use other commercial as well as self prepared standards (e.g. BaSO4). Possibility to restore factory calibration settings.
Following calibration kits are available on request:
- CKG16 for glass tubes with diameter 16 mm, set of 0.5; 1.0; 2.0; 3.0; 4.0 McFarland Turbidity Standards (latex particles);
- CKG1802 for glass tubes with diameter 18 mm, set of 0.5; 1.0; 2.0; 3.0; 4.0; 5.0 McFarland Turbidity Standards (BaSO4);
- CKG12 for glass tubes with diameter 12 mm, set of 0.0 (blank); 0.5; 2.0; 3.0 McFarland Turbidity Standards (latex particles).
Two versions of the product are available:
- DEN-1 powered from external energy supply;
- DEN-1B powered both from external energy supply and from batteries (АА). Besides, DEN-1B operates with higher precision of measurements (up to 0,01 McF).
|Measurement range||0.00–15.00 McF|
|Display resolution||0.01 McF|
|Measurement wavelength (λ)||λ = 565 ±15 nm|
|Accuracy (0.0–6.0 McF)||±3%|
|Measurement time||1 s|
|Sample volume||not less than 2 ml|
|Tube external diameter||12 mm, 16 mm (using A-12, A-16 adapter) or 18 mm (without adapter)|
|Possibility to restore factory calibration settings||+|
|Independent power supply||3 × AA batteries|
|Overall dimensions (W×D×H)||165 × 115 × 75 mm|
|Input current/power consumption||12 V, 7 mA / 0.1 W|
|External power supply||Input AC 100–240 V, 50/60 Hz; Output DC 12 V|
|Standard set||External power supply, A-16 and 3 × AA batteries|
Glass sample tubes without lid (16х100x0.8mm), 78 pcs. Fits DEN-1, DEN-1B factory calibrated
CKG16 for glass tubes with diameter 16 mm, set of 0.5; 1.0; 2.0; 3.0; 4.0 McFarland Turbidity Standards (latex particles).
McFarland Turbidity Standards, Ø18mm
McFarland Turbidity Standards, Ø12mm
A-12, adapter for work with tubes which are 12 mm in external diameter.