Description
For additional information
ask a questionHigh–Speed Multi Plate Shaker MPS-1 can be used in virtually any application by providing adjustable mixing of reagents in microtest plates, PCR plates, deepwell plates and test tubes (shaking tubes 0.2 to 2 ml and vortexing any volume up to 50 ml).
The shaker is compact and user–friendly. The shaker is ideal for personal use.
MPS-1 features a head for vortexing a single tube.
Shaker can be used in cold rooms or incubators, operating at ambient temperature range +4°C to +40°C. Low voltage external power supply (12 V) provides electrical safety in humid environment.
MPS-1 features Pulse Mode mixing function that works on the principle of giving a periodic impulse: the tube is accelerated to the set speed, holds it for 3 seconds and then drops the speed to zero. This motion is repeated until the timer runs out. This method provides a constant state of resuspension of the particles inside a tube, as the acceleration is always changing. The advantage of this method is the high throughput of mixed samples compared to vortexing a single tube.
Features:
- Speed control range 300–3,200 rpm
- Smooth mixing with 3 mm orbit
- Mixing presets
- Pulse Mode mixing function
- Quiet operation — low noise at maximum speed
- High stability — stationary at maximum speed
- Universal platform holder for Deepwell plates and Microtest plates
- Additional platforms for semi- and unskirted PCR plates 200 μl as well as for tubes from 0.2 to 2 ml
- Can be used in cold rooms or incubators (temperature range +4°C to +40°C)
Specification
| Mixing speed control range | 300–3,200 rpm (100 rpm increment) |
|---|---|
| Universal platform for deepwell plates, 96-well microtest plates (U, V or flat bottomed), 384-well microtest plates | + |
| Types of mixing presets: | -> |
| VORTEX | 3,200 rpm |
| HARD | 2,600 rpm |
| MEDIUM | 1,800 rpm |
| SOFT | 1,000 rpm |
| CUSTOM | adjustable rpm |
| Digital time setting | 0–60 min (15 s increment) / non–stop |
| Timer sound signal | + |
| Maximum continuous operation time | 8 h |
| Orbit | 3 mm |
| Acceleration time to maximum speed | 5 sec |
| Maximum load | 0.3 kg |
| Features a Pulse Mode mixing function | + |
| Features a Vortex function | + |
| Noise level, not more | 65 dB |
| Overall dimensions (W×D×H) | 225 × 215 × 150 mm |
| Weight | 5.1 kg |
| Input current/power consumption | 12 V, 800 mA / 10 W |
| External power supply | Input AC 100–240 V; 50/60 Hz; Output DC 12 V |
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Why do we need new mixing principle in biotechnology - in fact there already are various shakers for flasks, disposable bags with wave mixing technology, falcon tubes with permeable gas membranes and bioreactors with various mixing principles?
You are correct, in reality, there are many types of reaction vessels and bioreactors that the market can offer for the cultivation of microorganisms, yet we think that RTS technology has its unique niche.
The most important issue when choosing a bioprocess technology is whether it is scalable. The impossibility of direct transfer of optimal conditions from thermoshakers to bioreactors became a common issue. Many engineers are in search for the middle ground, which is so that the cultivation in the bioreactor would be as simple and accessible as in shaker flasks and so that the process could be carried out in parallel from one hand, and on the other hand so that the bioprocess would be scalable. Lower aeration values using shaker flasks in comparison to bioreactors or, in other words, low oxygen mass transfer into the culture medium does not allow getting as high cell densities as quickly as possible like in bioreactors.
What other noninvasive mixing principles that are available on the market today?
As can be observed from image. Biosan produces most of devices for noninvasive mixing which in turn can be used for bioprocesses. Moreover, we would like to introduce a new mixing principle into this table called Reverse Spinning. In the future, we will develop multi-channel RTS devices with higher and smaller volumes. In our estimates, the mixing technology can be scaled up to 20 litres and scaled down to 5ml. With a possibility to add vital bioprocess parameters noninvasive measurement such as pH and pO2
What is the mixing principle of RTS technology devices?
Initiation of the Vortex Type Mixing (VTM) and depth of the Vortex cave depend on 1) angular speed of RS–Reactor 2) time from initiating rotation of RS–Reactor 3) growth media viscosity 4) temperature. These parameters, also, determine the angular speed of rotating Vortex Layer (VL) and transition state from the Irrotational Vortex (IRV), when angular speed of the VL is proportional to the radius, to the Rotational Vortex, when the angular speed of the VL is the same and VL looks like a monolithic Vortex cavity, as shown below. Common rules regulating VTM processes may be stated as follows: the more time has passed since Vortex formation, the more obvious is a transition from IRV to the RV. The concept of the Reverse–Spin (RS) mixing is based on these assumptions.
What is the measurement principle of RTS technology devices?
What is the measurable range of biomass in OD600nm units?The essential elements of RTS-1 measurement system consist, as shown below, of a light source, a detector (photodiode), a falcon tube compartment in a rotating state. The falcon tube accelerates to 2000 min-1 creating a monolayer of cell suspension shortening the optical path of the sample. By shortening the optical path, it is possible to measure biomass in the range of 0-19 OD600nm at 10-20ml and 0-15.2 OD600nm at 10-30ml.
Thin layer increases OD measurement by mechanical non-invasive dilution.
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