Nucleic acid sequence-based amplification (NASBA) is an isothermal method able to specifically amplify target RNA in a DNA background. By combining NASBA amplification with molecular beacon probes, this assay becomes a Real-Time analyses tool that offers faster results compared to quantitative PCR (qPCR). This, along with its isothermal nature, makes NASBA ideal for remote monitoring and/or high-throughput applications.
NASBA has been applied as commercial diagnostic tests for the detection and/or quantitation of number of pathogens including C. trachomatis, HIV, Hepatitis C virus.
See the video from Bioforum 2011, where we demonstrated NASBA LIVE.
Download Article (PDF)