Densitometer is designed for measurement of cell suspension's turbidity in the range of 0.3–5.0 McFarland units (100×106 – 150×107 cells/ml).
Densitometer provides the opportunity to measure solution turbidity in a wider range (up to 15.0 McFarland units) however, it is necessary to remember that in this case the standard deviation values increase.
A densitometer is used for measurement of cell concentration (bacterial, yeast cells) during fermentation process, determination of microorganism sensitivity to antibiotics, microorganism identification using various test-systems, for measurement of absorption at the definite wavelength, as well as for quantitative estimation of concentration of colour solution, absorbing green light.
The operation principle is based on measurement of optical density with digital presentation of results in McFarland units. The unit is calibrated at the factory (for operation with 16 mm diameter glass tubes) and keeps calibration without power supply. However, if necessary it is possible to calibrate the unit by 2–6 points in 0.5–5.0 McFarland unit range. We recommend to use Biosan standards to ensure full reliability, but it is acceptable to use other commercial as well as self prepared standards (e.g. BaSO4). Possibility to restore factory calibration settings*
Following calibration kits are available on request:
Two versions of the product are available:
*NEW FUNCTION
| Light source | LED |
| Measurement wavelength (λ) | λ = 565 ±15 nm |
| Measurement range | 0.3–15.0 McF |
| Display resolution | 0.1 McF |
| Accuracy (0.3–5.0 McF) | ±3% |
| Measurement time | 1 s |
| Sample volume | not less than 2 ml |
| Tube external diameter | 16 mm (using A-16 adapter) or 18 mm (without adapter) |
| Possibility to restore factory calibration settings | + |
| Display | LED |
| Overall dimensions (W×D×H) | 165 × 115 × 75 mm |
| Weight | 0.7 kg |
| Input current/power consumption | 12 V, 80 mA / 1 W |
| External power supply | Input AC 100–240 V, 50/60 Hz; Output DC 12 V |
| Standard set | External power supply, A-16 adapter for ø16mm tubes |
Yes. Glass and transparent plastic tubes (16 or 18 mm in external diameter) can be used for work with DEN-1 densitometer. A-16 adapter (included) must be used for work with tubes which are 16 mm in external diameter.
Note! The unit must be calibrated every time a tube type (e.g. with different ext. diameter, bottom shape or different material) is changed.
Yes. Both commercial standards and standards prepared in the laboratory can be used for calibration.
From December 2015 factory calibration is for work with glass tubes with an external dimeter of 16 mm
Calibration procedure is described in the user manual p.3.4. See user manual below.
Procedure of restoration of factory calibration settings is described in the user manual p 3.5. See user manual below.
